|
KMID : 0606920170250040427
|
|
Biomolecules & Therapeutics
2017 Volume.25 No. 4 p.427 ~ p.433
|
|
|
Galangin Activates the ERK/AKT-Driven Nrf2 Signaling Pathway to Increase the Level of Reduced Glutathione in Human Keratinocytes
|
|
Hewage Susara Ruwan Kumara Madduma
Piao Mei Jing
Kang Kyoung-Ah
Ryu Yea-Seong
Fernando Pattage Madushan Dilhara Jayatissa
Oh Min-Chang
Park Jeong-Eon
Shilnikova Kristina
Moon Yu-Jin
Shin Dae-O
Hyun Jin-Won
|
|
|
Abstract
|
|
|
|
Previously, we demonstrated that galangin (3,5,7-trihydroxyflavone) protects human keratinocytes against ultraviolet B (UVB)-induced oxidative damage. In this study, we investigated the effect of galangin on induction of antioxidant enzymes involved in synthesis of reduced glutathione (GSH), and investigated the associated upstream signaling cascades. By activating nuclear factor-erythroid 2-related factor (Nrf2), galangin treatment significantly increased expression of glutamate-cysteine ligase catalytic subunit (GCLC) and glutathione synthetase (GSS). This activation of Nrf2 depended on extracellular signal-regulated kinases (ERKs) and protein kinase B (AKT) signaling. Inhibition of GSH in galangin-treated cells attenuated the protective effect of galangin against the deleterious effects of UVB. Our results reveal that galangin protects human keratinocytes by activating ERK/AKT-Nrf2, leading to elevated expression of GSH-synthesizing enzymes.
|
|
|
KEYWORD
|
|
|
Galangin, Reduced glutathione, Nuclear factor-erythroid 2-related factor, Glutamate-cysteine ligase catalytic subunit, Glutathione synthetase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|